Mycobacterium is a genus of nonmotile, non–spore-forming, aerobic bacilli in the Mycobacteriaceae family. They are slightly curved rods with filamentous and branching forms on occasion. They form mould-like pellicle in liquid medium; thus, the name…
Many mycobacterial culture samples are heavily contaminated with commensal flora. It is critical to remove these because they can outgrow the culture faster than the mycobacteria. Principle of the Petroff’s Concentration Method Contaminant destruction is…
Lowenstein Jensen medium is recommended for the isolation and cultivation of Mycobacterium species. It was originally formulated by Lowenstein, which included dyes such as congo red and malachite green. Jensen altered Lowenstein’s medium by changing…
This test is used to distinguish acid-fast bacteria based on their appearance after staining with acid-fast stain containing 20% sulphuric acid (Ziehl-Neelsen stain) as a decolorizer. Principle of Ziehl-Neelsen stain Because of the high lipid…
