Koh-Calcofluor White Stain is used to detect the presence of Acanthamoeba fungal elements and cysts in clinical specimens. Principle of Koh-Calcofluor White Stain The alkali potassium hydroxide (10 percent KOH) aids in the digestion of…
Many mycobacterial culture samples are heavily contaminated with commensal flora. It is critical to remove these because they can outgrow the culture faster than the mycobacteria. Principle of the Petroff’s Concentration Method Contaminant destruction is…
This test is used to distinguish acid-fast bacteria based on their appearance after staining with acid-fast stain containing 20% sulphuric acid (Ziehl-Neelsen stain) as a decolorizer. Principle of Ziehl-Neelsen stain Because of the high lipid…
Lactophenol cotton blue stain is used to identify fungi grown on culture from any clinical material. Principle of Lactophenol cotton blue stain The stain is used to stain fungus grown on ‘slide culture technique’ or…
The purpose of Gram staining is to demonstrate the presence of Gram positive and Gram negative bacteria in clinical specimens using morphology and Gram staining. This test is useful for determining the presence of potentially…
