Kobayashi et al. created TCBS Agar by modifying Nakanishi’s selective medium. Although this medium was designed to isolate V.cholerae and V. parahaemolyticus, most Vibrio’s grow to healthy large colonies with a variety of colonial morphologies….
Clinical materials containing a small number of Vibrio should be inoculated into an enrichment medium before plating onto a selective medium, such as TCBS Agar. Alkaline Peptone Water is an excellent enrichment broth for Vibrio…
The ability to reduce nitrate is useful for differentiating and identifying different types of bacteria, particularly those in the Enterobacteriaceae family. The ability of non-fermenters and other gram-negative bacilli to reduce nitrates varies. Some members…
Lowenstein Jensen medium is recommended for the isolation and cultivation of Mycobacterium species. It was originally formulated by Lowenstein, which included dyes such as congo red and malachite green. Jensen altered Lowenstein’s medium by changing…
Dermatophytes are a distinct group of fungi that infect human and animal hair, skin, and nails, causing a variety of cutaneous infections known as ringworm. Dermatophytes such as Trichophyton, Microsporum, and Epidermatophyton cause the majority…
The clinical laboratory has access to a variety of enrichment and selective media for the isolation and identification of pathogenic fungi. The majority of the media mentioned in this article are commercially available in dehydrated…
Sabouraud Dextrose Agar is a culture medium for yeasts, molds, and aciduric microorganisms. Sabouraud Dextrose Agar is Carlier’s modification of Sabouraud’s formulation for the cultivation of fungi (yeasts, molds), which is especially useful for fungi…
Brain Heat Infusion Broth is highly nutritious and is recommended for the propagation of pathogenic cocci and other fastidious organisms associated with blood culture work and allied pathological investigations, as well as for Staphylococcus aureus…
